Beckman Red Cell Lysis, beckm Red Cell Lysis, beckman Cell Lysis, Beckman cikakken jini Red Cell Lysis
Bayani:Versalyse Lysing Solution,100tests

Mai ba da: Guangzhou North Rui Chromatography Technology Co., Ltd.

Don sauran nau'ikan reagents, don Allah kira mu kamfanin don bincike.

Red Cell LysateHanya ce mai sauƙi don cire red blood cells. Wasu ƙwayoyin halitta masu lalacewa suna da ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta masu ƙwayoyin halitta. A cikin ƙwayoyin ƙwayoyin da aka samu ta hanyar ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙway

Red ƙwayoyin cutaKa'idar

Akwai antigen na farfajiyar jiki na jiki na jiki a farfajiyar jiki. Lokacin da ruwan ya ƙunshi enzymes da za su iya kai hari kan takamaiman antigen na jiki na jiki na jiki, kawai zai haifar da karkatarwar jiki na jiki, hanyoyin halitta suna fadada, fadada, rarrabuwa, ba tare da kai hari kan sauran ƙwayoyin halitta ba. Bugu da ƙari, ja ƙwayoyin cuta suna da nasu electronegativity, penetration rauni, dakatarwa kwanciyar hankali, wannan ne bambanci daga sauran nau'ikan ƙwayoyin cuta. Red cell lysis yana amfani da waɗannan halaye don rarraba red cells.

Red Cell Lysateumarnin amfani

1. 2x girman sabo cikakken jini, ƙara 3x girman red cell lysis. Ƙara 3ml kamar 1ml sabo cikakken jiniRed Cell LysateA hankali juyawa ko juyawa. 2. An sanya shi a kan kankara na minti 15, a tsakanin haka an haɗa shi sau biyu a hankali, bayan da ja ƙwayoyin cuta suka faɗaɗa, mafita ya kamata ya kasance mai haske da haske. 3. Tattara kwayoyin halitta: 4 ℃, 450 × g centrifuge for mintuna 10 don precipitate farin ƙwayoyin halitta, da hankali a tsotse da subliminal ruwa. 4. Ƙara sau biyu da girma na red cell lysis a cikin fararen ƙwayoyin cuta da kuma sauƙi vortex cikakken sake dakatar da fararen ƙwayoyin cuta. Idan jinin farawa shine 1ml, a ƙara 2ml na red cell lysis. 5. 4 ℃, 450 × g centrifuge don mintuna 10 da aka zubar da farin ƙwayoyin cuta, da hankali da kuma cikakken suck a kan clearance ruwa. 6. sake dakatar da ƙwayoyin don binciken gwaje-gwaje; Kamar yadda aka cire RNA, * a wannan mataki fara aiki tare da DEPC ruwa samar da mafita.

Red Cell LysateHanyoyin saiti:

Dauki 100 μl anti-coagulation cikakken jini, ƙara 2 ml aikace-aikace ruwa 3 #, hada 15 ~ 20 s; ƙara 2 ml aikace-aikace ruwa 2 #, hada 15 ~ 20 s; ƙara 4 ml aikace-aikace ruwa 1 #, hada 15 ~ 20 s; 300 × g centrifuge 2 min, zubar da sama tsabtace; Tattara ruwan sama, ƙara PBS buffer, yin farin ƙwayoyin cuta dakatarwa, ajiya.

(Wannan red cell fission hanyar sadu da farin ƙwayoyin cuta Flow Cytometry ganowa rarraba bukatun, low farashi, shi ne mai sauki da sauri, tattalin arziki da kuma amfani da farin ƙwayoyin cuta tsarkakewa rarraba hanyar.)

Red Cell LysateKulawar

1, wannan fissile ne sterile kayayyakin, raba kwayoyin halitta don amfani da kwayoyin halitta al'ada don Allah kula da sterile aiki.

2. Don lafiyarku da lafiyarku, don Allah sa tufafin gwaji da kuma sa hannuwar hannuwar aiki.

0x ajiya ruwa ajiya a 2-8 ° C ba zai iya wuce rabin shekara (watanni shida);

4, a lokacin amfani da daidai da bukatar, a kowane lokaci da ake amfani da ruwan deionization sabo shirye-shiryen aiki ruwa, a ranar da ake amfani da shi ba za a iya zubar da shi ba.