Bayanin samfurin:

CE ISO 18979-2003Misalin tryptoxin a cikin abinci Tsabtaccen amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi na amincin rigakafi—High inganci ruwa chromatography da kuma immune affinity layering tsabtace—Fluorescence gauge yanayin da kuma cikakken bincike matakai na tryptoxin a abinci

Wannan ƙa'idar tana amfani da ƙididdigar tryptoxin a cikin masara, walungu da kayayyakinsa (walungu, walungu, walungu), shinkafa, alkama, man shuka, soya sauce, cin abinci da sauransu.
Iyakin ganowa na tryptoxin a samfurin: tsarkakewa na immune affinity—High inganci ruwa chromatography da kuma immune affinity layering tsabtace—Fluorescence matakan1mg / kg; tsarkakewa na immune affinity—Fluorescence gauge na soya sauce samfurin ganowa na yellow mycotoxin iyakance zuwa2.5mg / kg.
Immunoaffinity Layering tsabtace High inganci ruwa chromatography
Hanyar Bayani
samfurin ta hanyar methanol-Ruwa cirewa, cirewa ruwa ta hanyar tacewa, rarraba, tacewa ta hanyar tsarkakewa ta hanyar rigakafi affinity stratification dauke da musamman kwayoyin cuta na tryptoxin, wannan kwayoyin cuta na tryptoxinB1kumaB2kumaG1kumaG2Tare da musamman, tryptoxin crosslinked a kan antibodies a cikin layered kafofin watsa labarai. Ta ruwa ko Twain/ PBSAn cire ƙarancin da ke kan ginshikin rigakafi, an wanke shi da methanol ta hanyar ginshikin rigakafi, kuma an auna abun ciki na tryptoxin ta hanyar samar da bayan iodine mafita ta hanyar ingantaccen ruwa chromatography ginshikin tare da na'urar ganowa.
Kayan aiki da kayan aiki
Lab yau da kullun kayan aiki, kayan aiki, kayan da wadannan abubuwa.
1 High gudun homogenizer:18,000 r / min ~ 22,000 r / min.
2 Yellowmycin rigakafi affinity ginshike.
3 Fiberglass tace takarda: diamita10cm, diamita1.5μm.
4 Glass allura:10 ml，20 ml.
5 Glass gwajin bututun: diamita12 mmtsawo75mmBabu wani fluorescent halaye.
6 High inganci ruwa chromatography: tare da360nmmotsawa wavelengths da kuma mafi girma fiye da420nmFluorescent detector na watsa wavelengths.
7 Air matsin lamba famfo.
8 Trace allura:100ml.
9 Tsarin Chromatography:C18tsawon ginshike150 mm, ciki diamita4.6mmDiamita na cikawa5 mm).