TIRF、 mayar da hankali, FRET、 Hasken haske da fasahar allura ta microscopy sun taimaka wa masana kimiyya su shawo kan matsaloli da yawa a cikin hoton ƙwayoyin halitta. Ti ne zuciyar dukan fasahar, tare da wannan sabon nau'in mai ƙarfi mai juyawa, za ka iya yin amfani da shi a Nikon CFI60 ® Sauƙin amfani da fasahohin da aka ambata a sama tare da taimakon tsarin gani. Jerin Ti yana da samfuran uku, ingantaccen saurin tsarin, haɓaka sassauci da ingantaccen fasali mai yawa ya sa Ti ya zama tsarin da ya dace don bincike da hoton ƙwayoyin halitta.

Nikon bincike matakin juyawa biomicroscope

High quality bambanci image

Nikon reverse microscope Eclipse Ti-E / Ti-U / Ti-S mai zane na gani ya haɓaka rukunin bambanci na waje. Ta amfani da wannan tsarin da aka haɗa zoben bambanci a cikin jikin microscope maimakon abubuwa, masu amfani ba dole ne su yi amfani da abubuwan bambanci don kallon hotunan bambanci ba, kuma za su iya samun hotuna masu inganci ta hanyar abubuwan da ke da ƙididdiga mai girma. Hakanan amfani da abubuwa ba tare da zoben bambanci ba zai iya samun hoton fluorescent na "cikakken haske".

Bambancin zobe da aka sanya a cikin jikin microscope

Tsarin hanyar haske na bambancin bambancin bambancin da aka sanya a cikin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin Dangane da abubuwan da aka yi amfani da su, akwai nau'ikan zoben bambanci guda huɗu (Ti-E / U / S Universal).

Ultra high ƙuduri

Amfani da Nikon's high-performance abubuwa, ciki har da 60x da 100xTIRF abubuwa, tare da 1.49 lambar aperture, da kuma hadewa da bambancin gyara zoben, za a iya samun babban ƙuduri bambancin hoto na wasu misali bambancin abubuwa *.

"Cikakken haske" hoton fluorescent da aka samu ta amfani da abu guda daya Saboda hasken haske wanda babu bambancin zoben, a cikin tsarin guda daya, ba kawai za a iya lura da bambancin ba, har ma za a iya samun hotuna masu haske na "cikakken haske", hotuna masu mayar da hankali da hotuna na TIRF.(Tushen: Consistent kayan aiki)

Duba bambancin hotuna ta amfani da ruwan ruwa

Ta hanyar na'urorin bambanci na waje, za a iya samun hoton bambanci mai haske da ƙuduri har ma da amfani da ruwan ruwa.

Hotuna masu ƙuduri don nazarin hoto

Saboda bambancin hotuna da TIRF lura, DIC lura za a iya amfani da iri ɗaya abubuwa, da kuma samun hotuna za a iya amfani da high daidaito data sarrafawa da kuma image nazarin, misali, TIRF hotuna da kwayoyin halitta profile ma'anar.

Multi tashar jiragen ruwa hierarchical goyon bayan bincike

Nikon reverse microscope Ti-e yana da tashar jiragen ruwa mai yawa tare da tashar jiragen ruwa ta hagu, tashar jiragen ruwa ta dama da tashar jiragen ruwa ta ƙasa * don haɗa kyamara ɗaya a kowane tashar jiragen ruwa. Bugu da ƙari, tsarin sararin samaniya na layered yana iya ƙara tashar jirgin ruwa ta baya, waɗannan halaye suna sauƙaƙe masu amfani da akwatin matattarar launi mai layi biyu da kyamarori da yawa don samun hoto. * Ti-E / B da Ti-U / B Combined zaɓi ƙasa tashar jiragen ruwa(Tushen: Consistent kayan aiki)

Bayan tashar jiragen ruwa tabbatar da Multi-kamara harbi

An faɗaɗa damar samun hoto ta amfani da ƙirar tashar jirgin ruwa ta zaɓi. Amfani tare da tashar jiragen ruwa ta gefe yana ba da damar samun hoton tashar biyu tare da kyamarori biyu. Misali, lokacin da akwai nisan kallo tsakanin furotin fluorescent na FRET (Foster Resonance Energy Transfer), bambancin ƙarfi tsakanin CFP da YFP yana da yawa, ana iya kwatanta hotuna masu yawa ta hanyar daidaita hankalin kyamarar ɗaya.

Layered tsari inganta scalability

Tsarin layered da Ti ya yi amfani da shi yana amfani da fa'idodin tsarin gani mai nisa mara iyaka kuma ya haɗa PFS zuwa masu canza abubuwa. Za a iya gabatar da abubuwa biyu na zaɓi ban da PFS a cikin hanyar haske ta hanyar pad high block, tare da tsarin za a iya amfani da laser pins, na'urorin kunnawa na haske da na'urorin fluorescence na sauka a lokaci guda. Kowane layer na lantarki fluorescent tace launi block akwatin za a iya sarrafa daban.(Tushen: Consistent kayan aiki)

Samun hotuna da yawa fluorescent launi tare da mafi kyawun aiki a cikin wani wider wavelengths kewayon

Nikon reverse microscope Ti-U Ta hanyar gabatar da 870nm wavelength block na'urar, masu bincike za su iya amfani da kusan infrared fluorescent launuka ciki har da Cy5.5. An haɓaka halayen gani daga ultraviolet zuwa infrared, yawan abubuwan da ke akwai yana ƙaruwa, yana ba da damar mai da hankali a cikin aikace-aikace masu yawa, ko a cikin Ca na ultraviolet²⁺Matsayi auna ko infrared kewayon laser pins.

Musamman sauri image samu

Yin hanyoyi uku (fluorescence da bambanci na hanyoyi biyu) a kan allon rami 96 da sauri, da sauri fiye da sau biyu.

Nikon* ta cikakken daidaitawa tsarin (PFS) kawar da mayar da hankali yawo

Mai mayar da hankali yana da babban shinge a cikin lura da jerin lokaci. Tsarin PFS na Nikon yana gyara mai mayar da hankali da zai iya faruwa yayin dogon lokacin lura da kuma lokacin da aka saka. Za a iya kiyaye mayar da hankali ko da yana amfani da abubuwan da ke da ƙarfi ko fasahohi kamar TIRF. Bugu da ƙari, haɗawa da PFS a kan mai juyawa na abu yana taimakawa wajen adana sarari kuma ba ya iyakance tsarin layered mai girma na Ti. PFS yana amfani da ingantaccen tsarin diyya na gani don daidaitawa a ainihin lokacin Z axis. Lokacin da ba a buƙatar amfani da PFS ba, za a iya cire shi daga hanyar haske.

Digital sarrafawa cibiyar haɓaka gudun lantarki kayan haɗi

Nikon bincike matakin juyawa microscope Eclipse Ti-E / Ti-U / Ti-S sabon ci gaba da dijital sarrafa cibiyar ta hanyar rage sadarwa lokaci tsakanin sassa, inganta saurin daban-daban kayan haɗi, da kuma muhimmanci inganta gaba ɗaya aiki gudun. PC sarrafawa inganta lantarki sassa na Ti, rage amsa lokaci tsakanin aiki umarnin zuwa motsi, don haka yi babban gudun sarrafawa a gaba ɗaya. Ta hanyar ƙara firmware mai hankali, jimlar lokacin aiki na sassan lantarki ya rage sosai, misali, jimlar lokacin da ake buƙata don samun hoto mai ci gaba da tashoshi uku (fluorescence mai tashoshi biyu da bambanci) ya rage sosai, yana rage gubar haske ga ƙwayoyin halitta.

High gudun lantarki iko da image samu

Nikon juyawa microscope Ti-S daidaita sarrafa da dama lantarki sassa, kamar abubuwa canza, fluorescent tace block, haske lattice, mai mayar da hankali da kuma kaya tebur, masu bincike iya gudanar da multi-girma lantarki gwaji. Saurin motsi na kayan haɗi da samun hoto yana rage lokacin fallasa gaba ɗaya, rage hasken haske mai dacewa, yana taimaka wa masu bincike su sami ƙarin ma'ana.

Ƙara gudun kowane lantarki sassa

Saurin aiki da / ko canza abubuwa, tace block, XY daukar kaya, motsawa / toshe tace ya karu sosai, kuma masu bincike za su iya mai da hankali kan lura da kuma samun hoto. Sabon mai kula da aka haɓaka zai iya yin rikodin da kwafin yanayin lura, yana amfani da linzamin kwamfuta don sarrafa tashar ɗaukar kaya, kuma dukan microscope yana kama da ɓangarorin ido da hannun mai bincike.(Tushen: Consistent kayan aiki)

Kowane hanyar lura tana amfani da ingantaccen fasahar gani don samun cikakken hoto

Fasahar gani da Nikon ta inganta tana ba da hanyoyi da yawa don lura da samfurin, yana nuna wa masu bincike kowane cikakken bayani game da ƙwayoyin.

Nomarskibambanci tsoma baki (DIC)

Daidaitaccen bambanci mai girma da ƙuduri mai girma yana da mahimmanci don lura da microstructures. Tsarin DIC na Nikon* yana samun hotuna masu ƙuduri har ma a ƙananan ƙarancin girma. Sabon DIC slider (bushewa) yana ba da zaɓuɓɓuka biyu na babban ƙuduri da babban bambanci. Ana iya sanya DIC daukar hoto a cikin akwatin tacewa na lantarki, yana rage lokacin canzawa na DIC da kuma lura da fluorescence.

bambanci

Ana iya lura da bambancin hoto ta hanyar amfani da CFI Plan Fluor ADH 100x (Man fetur). Wannan abu yana rage halo na bambancin hoto idan aka kwatanta da abubuwan bambancin gargajiya, yana haɓaka bambancin hoto.

Dark Field

Ana iya yin lura da filin duhu ta amfani da matattarar da ke da babban NA. Ana iya lura da microparticles na dogon lokaci kuma kauce wa haske whitening.

Hoffman daidaitawa bambanci (HMC) ®

HMC abubuwa tare da HMC focusing kayan haɗin za a iya samun high bambanci, halo-free hoto kamar 3D, wanda za a iya amfani da shi don noma m samfurin a cikin filastik Petri kwanoni.

Sabbin abubuwan da aka haɓaka don jerin Ti

CFI S Shirin Fluor ELWD/ELWDbambancin abubuwa

Sabon abubuwan da aka haɓaka suna da babban ƙwarewa ga haske a cikin kewayon kusan ultraviolet (Ca2 +) zuwa kusan infrared, kuma suna inganta gyaran bambancin launi. Ana iya samun hotuna masu inganci a yanayin haske daban-daban.

Shirya Apochromat 20xAbubuwan

Sabon abu na 20x ya haɗu da jerin abubuwan VC na Nikon, wanda aka gyara bambancin launi na axial zuwa 405nm, wanda ya zama kyakkyawan abu don kulawa mai mayar da hankali da fasahar kunnawa ta haske.

Inganta operability

Duk maɓallin maɓallin da sarrafawa masu canzawa don aikin lantarki suna da ƙirar mutum sosai, kuma masu bincike za su iya mai da hankali kan bincike ba tare da tasirin aikin microscope ba.

Operation maɓallin ne a gefen da kuma gaban jikin microscope

Switching na fluorescent tace block, abu canji, Z axis thickness / daidaitawa, PFS kunna / kashe iko, watsa haske kunna / kashe iko duk za a iya sauya da sauri ta hanyar maɓallin da ke cikin microscope jiki.(Tushen: Consistent kayan aiki)

New R & D mutum-inji mai kula

Ta hanyar hannu ko mutum-inji mai sarrafawa za a iya sarrafa high-gudun lantarki XY daukar tebur da Z axis.

VFD allon gaban jikin microscope da kuma aiki maɓallin

Yanayin microscope, ciki har da bayanan abu, da kuma yanayin kunna / kashewa na PFS duka suna nunawa a allon VFD. Tune.

PFSCompensation ayyuka

PFS ta biyan kuɗi aiki ne mai sauƙi sarrafawa, kawai daya maɓallin zai iya canzawa m / daidaitawa.

Nisa iko panel da kuma tsoho maɓallin

Nikon Inverted Bio Microscope Eclipse Ti-E/Ti-U/Ti-S yana iya sarrafa microscope ta hanyar bangaren sarrafawa na nesa kuma ya tabbatar da yanayin microscope na yanzu. Hakanan ana iya canzawa yanayin lura ta atomatik ta hanyar maɓallin tsoho. Sauyawa daga bambanci zuwa lura da fluorescence za a iya kammala da maɓallin guda ɗaya.

Original karkata zane

An karkata gaban jikin microscope ɗan ɗan baya, yana rage nisan da ke tsakanin idanun mai aiki da samfurin kimanin 40mm, yana haɓaka aiki.

Nikon juyawa microscope Ti-Efasaha sigogi

Nikon juyawa microscope Ti-Ufasaha sigogi

Nikon juyawa microscope Ti-Sfasaha sigogi

Nikon bincike matakin juyawa biomicroscopeGirman: