GENECLEAN ® Ka'idar Turbo ita ce haɗuwa, tsabtace da wanke. Ta amfani da musamman haɗuwa hanyar, GlassMilk fifiko haɗuwa da nucleic acid a ƙarƙashin high gishiri low pH muhalli. Sa'an nan kuma an haɗa sassan ta hanyar sauki tsabtace, wanke gonadotropin daga tsarin tsabtace, ƙuntatawa endocytase, Taq enzyme, T4 connectase, primer, junction, gishiri da sauran organic solvents da sauransu. A ƙarshe, an wanke kwayoyin DNA ta amfani da ƙarancin gishiri ko ruwa, kuma an tsarkake DNA kai tsaye don gwaje-gwaje masu zuwa.

Abubuwan amfani:

GENECLEAN ® Turbo hanyar da core fasaha shi ne da GlassMilk kunshin a Turbo membrane (patent kariya), rage da m Glassmilk centrifugal tsari haifar da membrane karya. Turbo membrane da aka saka a kasa na ginshiƙin centrifugal yana sauƙaƙa matakai masu nauyi yayin tsarkake DNA na Glassmilk mai laushi, kuma tsarkakewar tsarkakewar ethanol ya haɓaka yawan dawo da DNA da tsarki. Hakanan idan aka kwatanta da sauran tsarin tsarkakewa na tushen membrane, Glassmilk mai siffar da ba daidai ba, da kuma kauri na Turbo membrane kanta, yana ba shi mafi girman yankin haɗuwa da ƙarin ƙarfi, saboda haka sake dawo da DNA ya kai 10 ug.

1, farawa kayan iyakancewa kananan: gonadotropin gel da TAE ko TBE buffer shirya, PCR amsa samfurin ko sauran enzyme amsa mafita;

2, gwajin aiki mai sassauci da sauri: Za a iya dawo da DNA ta hanyar inji ko centrifugal, za a iya kammala tsabtace samfuran 12 a cikin minti 15;

3, DNA tsabtace kewayon: 100 bp ~ 300 kb;

4, DNA tsabtace inganci high: tsabtace kudi har zuwa 95%, tsabtace adadin har zuwa 10 ug;

5, tsabtace DNA m aikace-aikace: kusan duk downstream gwaje-gwaje ciki har da sequencing, clone, PCR、 Transformation, micro allura, transfection da kuma microarray bincike.

Bayani sigogi:

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